Waiting
Elaborazione accesso...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Immunology and Infection / Human Colonoid Monolayers to Study Interactions…
JoVE Journal
Immunology and Infection

This Contenuto is Open Access.

JoVE Journal Immunology and Infection
Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium
 

Human Colonoid Monolayers to Study Interactions Between Pathogens, Commensals, and Host Intestinal Epithelium

Article DOI: 10.3791/59357-v • 07:20 min • April 9th, 2019
April 9th, 2019 •
*1, *2, 1, 1
1Department of Internal Medicine, University of New Mexico Health Science Center, 2Department of Medicine, Division of Gastroenterology & Hepatology, Johns Hopkins University School of Medicine
* These authors contributed equally

Capitoli

Riepilogo
Automatic Translation

Here, we present a protocol to culture human enteroid or colonoid monolayers that have intact barrier function to study host epithelial-microbiota interactions at the cellular and biochemical level.

Tags

Keywords: Colonoid Monolayers Intestinal Epithelium Host-pathogen Interaction Mucus Biology Microbiome Inflammatory Bowel Disease 3D Organoid Culture Ex Vivo Studies Harvesting Solution Cell Suspension DMEM/F12 Medium Cell Plating Expansion Medium
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter