/

/

构建用于扩展视场单分子成像的高倾角扫描磁贴显微镜的指南

JoVE Journal
Bioingegneria

This content is Free Access.

JoVE Journal Bioingegneria

A Guide to Build a Highly Inclined Swept Tile Microscope for Extended Field-of-view Single-molecule Imaging

Please note that all translations are automatically generated. Click here for the English version.

构建用于扩展视场单分子成像的高倾角扫描磁贴显微镜的指南

DOI:

10.3791/59360-v

•

08:13 min

•

April 08, 2019

•

Jialei Tang, Chun-Hung Weng, Jeffrey B. Oleske, Kyu Young Han

¹CREOL, The College of Optics and Photonics,University of Central Florida, ²Andor Technology,Oxford Instruments

Capitoli

00:04Titolo
00:28Preliminary Setup
01:11Setting up the Detection Path
02:14Setting up the Excitation Path and the Cylindrical Lenses
04:59Testing Tile Imaging
05:53Highly Inclined Swept Tile (HIST) Imaging
06:52Results: Highly Inclined Swept Tile Microscopy of Labeled DNA and RNA
07:38Conclusion

Summary

Traduzione automatica

详细介绍了如何建立高倾斜的扫描瓷砖 (HIST) 显微镜及其在单分子成像中的应用。

Tags

Keywords: Single-molecule Imaging Extended Field-of-view Swept Tile Microscope Piezo Stage Collimated Light Source Dichroic Mirror Tube Lens Multi-band Pass Filter Fold-mirror Objective Lens Airy Disc

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

循环压力为生物反应器的设计体外主动脉心脏瓣膜的研究

调查纤维素酶与基于AFM的单分子力谱受体 - 配体系统

从体素知识：实用指南复电镜三维数据的分割

受体动力学通过单分子荧光显微镜的高分辨率时空分析

3d轨道跟踪的改进双光子显微镜之应用：细胞内的囊泡的跟踪

从快速荧光成像在分子扩散法对活细胞的膜在商业显微镜

微京尼平沉积技术的微模式化血管肌肉薄膜的扩展文化

单分子荧光显微镜在平面支持双层膜

使用一个荧光显微镜进行多种成像模式

纳米等离子体增强散射和低放大显微镜成像对肿瘤衍生外质体的定量

Read Article