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Visualisering af Germinosomes og den indre membran i Bacillus subtilis sporer
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Capitoli
- 00:04Titolo
- 00:34High Precision Coverslip and Glass Slide Preparation
- 01:17Fluorescent Microsphere and Spore Sample Mounting
- 02:32Fluorescent Microsphere and Spore Imaging
- 03:38FM4-64 Stained PS4150 Spore 3D-SIM Raw Image Reconstruction
- 05:00Image Analysis
- 06:25Results: Representative Germinosome and Inner Membrane Visualization
- 08:04Conclusion
Germinant receptor proteiner klynge i 'germinosomes' i den indre membran af Bacillus subtilis sporer. Vi beskriver en protokol bruger super opløsning mikroskopi og lysstofrør reporter proteiner til at visualisere germinosomes. Protokollen identificerer også spore indre membran domæner, der fortrinsvis farves med membran farvestof FM4-64.
Tags
GerminosomesInner MembraneBacillus Subtilis SporesSuper-resolution Structured Illumination Microscopy3D-SIMFluorescent Reporter ProteinsSpore MembranesLipid DomainsCoverslipsMicroscope SlidesFluorescent MicrospheresAgaroseUltrapure WaterGene FrameStructured Illumination MicroscopePoint Spread FunctionExcitation Wavelengths
