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Method to Obtain Pattern of Breathing in Senescent Mice through Unrestrained Barometric Plethysmography
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Capitoli
- 00:04Titolo
- 00:29Barometric Plethysmograph Chamber Calibration
- 01:46Metabolic Analyzer Calibration
- 04:18Unrestrained Barometric Plethysmography (UBP)
- 05:22Metabolism and Breathing Pattern Analyses
- 06:07Apneas and Augmented Breath Analysis
- 07:08Results: Representative Mouse Breathing Pattern Analyses
- 08:42Conclusion
Unrestrained barometric plethysmography is used to quantify the pattern of breathing in awake mice. We show that 15 s segments under a standardized protocol display similar values to an extended duration of quiet breathing. This methodology also allows for the quantification of apnea and augmented breaths during the first hour in the chamber.
