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基于传输的野武学型差分干涉对比显微镜对等离子体纳米粒子的光谱处理
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JoVE Journal Chimica
Performing Spectroscopy on Plasmonic Nanoparticles with Transmission-Based Nomarski-Type Differential Interference Contrast Microscopy
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基于传输的野武学型差分干涉对比显微镜对等离子体纳米粒子的光谱处理

DOI:
10.3791/59411-v

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08:54 min

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June 05, 2019

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1Department of Chemistry and Biochemistry,Ohio University

Capitoli

  • 00:04Titolo
  • 00:33Glass and Nanoparticle Sample Preparation
  • 02:03DIC Imaging Preparation
  • 03:22DIC Nanoparticle Sample Imaging
  • 04:41Data Analysis using ImageJ
  • 06:39Results: Spectroscopy of Plasmonic Nanoparticles
  • 08:08Conclusion

Summary

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该协议的目的是详细介绍一种经过验证的制备等离子体纳米颗粒样品的方法, 以及用差分干涉对比度 (DIC) 显微镜对其进行单粒光谱的方法。

Tags

Plasmonic NanoparticlesTransmission-based Nomarski-type Differential Interference Contrast MicroscopyDIC MicroscopyDark FieldLateral ResolutionDepth Of FieldGold NanoparticlesCentrifugationResuspensionDropcastingKohler IlluminationHigh MagnificationDe Senarmont Design

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