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An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells
JoVE Journal
Medicina
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JoVE Journal Medicina
An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

An Enzyme-free Method for Isolation and Expansion of Human Adipose-derived Mesenchymal Stem Cells

DOI:
10.3791/59419-v

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04:37 min

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December 16, 2019

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, , , ,
1Department of Medicine, Division of Hematology/Oncology, New Jersey Medical School,Rutgers Biomedical and Health Sciences, 2Rutgers School of Graduate Studies at New Jersey Medical School,Rutgers Biomedical and Health Sciences, 3Department of Surgery, Division of Plastic Surgery, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Capitoli

  • 00:04Titolo
  • 01:10Isolation of ASCs from Adipose Tissue
  • 02:37Culture of ASCs
  • 03:29Results: An Explant-based Enzyme-free Method of ASCs Isolation
  • 04:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol provides an enzyme-free method for isolating mesenchymal stem cells from abdominoplasty and lipoaspirate samples using an explant method. The absence of harsh enzymes or centrifugation steps provides for clinically relevant stem cells that can be used for studies in vitro or transferred back to the clinic.

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Keywords: Adipose-derived Mesenchymal Stem CellsEnzyme-free IsolationExplant MethodCell ExpansionTrypsinizationClinical ApplicationsResearch Applications

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