/
/
Sanntid i Vitro overvåking av Odorant reseptor aktivering av en Odorant i damp fase
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Molecular Genetics and Microbiology,Duke University Medical Center, 2Department of Biotechnology and Life Science,Tokyo University of Agriculture and Technology, 3Department of Neurobiology,Duke University Medical Center, 4Department of Mechanical Systems, Engineering,Tokyo University of Agriculture and Technology, 5Institute of Global Innovation Research,Tokyo University of Agriculture and Technology, 6Duke Institute for Brain Sciences,Duke University
Capitoli
- 00:04Titolo
- 00:42Hana3A Cells Culture
- 02:26Preparation of the Cells for Transfection
- 03:23Plasmid Transfection
- 05:01Substrate Incubation
- 06:07Odorant Stimulation and Data Analysis
- 08:07Results: Real-time Activation and Dose Dependent Responses
- 09:21Conclusion
Fysiologisk aktiveres odorant reseptorer av odorant molekyler inhalert i damp fase. Men benytter mest i vitro systemer flytende fase odorant stimulering. Her presenterer vi en metode som gjør at sanntid i vitro overvåking av odorant reseptor aktivisering på odorant stimulering i damp fase.
