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通过对Rho激酶活性的暂时抑制,增强人类诱导多能干细胞衍生心肌细胞的诱导
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Enhancing the Engraftment of Human Induced Pluripotent Stem Cell-derived Cardiomyocytes via a Transient Inhibition of Rho Kinase Activity
DOI:

08:00 min

July 10, 2019

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Capitoli

  • 00:04Titolo
  • 01:13hiPSC-CMs Purification, Small Molecule Pre-treatment, Myocardial Infarction, and Cell Transplantation
  • 03:42Calcium Transient and Contractility Recording
  • 05:21Results: Increased Engraftment of Transplanted hiPSC-CM Preconditioned with the ROCK Inhibitor Y-27632 in a Murine MI Model
  • 07:11Conclusion

Summary

Traduzione automatica

在该协议中,我们演示并阐述了如何使用人类诱导多能干细胞进行心肌细胞分化和纯化,并进一步说明如何利用Rho相关蛋白激酶抑制剂提高移植效率小鼠心肌梗死模型的预处理。

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