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En kolorimetrisk analyse af citrat syntase aktivitet i Drosophila melanogaster
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Biochimica
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JoVE Journal Biochimica
A Colorimetric Assay of Citrate Synthase Activity in Drosophila Melanogaster

En kolorimetrisk analyse af citrat syntase aktivitet i Drosophila melanogaster

DOI:
10.3791/59454-v

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04:30 min

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January 16, 2020

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, , ,
1Shanghai Diabetes Institute, Shanghai Key Laboratory of Diabetes Mellitus, Shanghai Clinical Center for Diabetes,Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 2Department of Anatomy of Physiology,Shanghai Jiao Tong University School of Medicine

Capitoli

  • 00:04Titolo
  • 00:38Colorimetric Citrate Synthase Activity Assay
  • 02:46Results: Effect of dRNF34 and dPGC-1 Knockdown on Citrate Synthase Activity
  • 03:38Conclusion

Summary

Traduzione automatica

Traduzione automatica

Vi præsenterer en protokol for en kolorimetrisk analyse af citrat syntase aktivitet til kvantificering af intakt mitokondrie masse i Drosophila væv homogenater.

Tags

Keywords: Citrate SynthaseColorimetric AssayDrosophila MelanogasterMitochondrial FunctionExtraction BufferProtein ConcentrationEnzymatic ActivityDRNF34DPGC-1

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