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Capitoli
- 00:04Titolo
- 00:53Bacterial Cultivation and Extraction of Genomic DNA
- 01:54Multiplex PCR Setup and Cycling Conditions
- 03:14PCR Amplicon Confirmation by Gel Electrophoresis
- 04:37Capillary Electrophoresis Setup and Run Conditions
- 06:06VNTR (Variable-number Tandem-repeat) Size Calling, Repeat Count Calculation and MLVA (Multi-locus Variable-number Tandem-repeat Analysis) Profiling
- 07:36Minimum Spanning Tree Cluster Analysis of MLVA Data
- 09:21Results: Gel Electrophoresis, Electropherograms, and Minimum Spanning Tree
- 10:09Conclusion
De multi-Locus variabele-aantal tandem-repeat analyse (MLVA) assay hier gepresenteerd maakt goedkope, robuuste en draagbare hoge-resolutie genotypering van de vis-pathogene bacterie Yersinia ruckeri. Beginnend bij zuivere culturen, gebruikt de assay multiplex PCR en capillaire elektroforese om tien-loci MLVA-profielen voor downstreamtoepassingen te produceren.
