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An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
An Advanced Murine Model for Nonalcoholic Steatohepatitis in Association with Type 2 Diabetes
DOI:

10:17 min

April 26, 2019

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Capitoli

  • 00:04Titolo
  • 00:58Adipose Tissue Digestion
  • 03:37Liver Tissue Digestion
  • 05:56Surface Staining and Flow Cytometric Analysis
  • 07:53Results: Representative Leukocyte Population Analysis in a Nonalcoholic Steatohepatitis Mouse Model
  • 09:46Conclusion

Summary

Traduzione automatica

A simple and reliable diet-induced rodent animal model for nonalcoholic steatohepatitis (NASH) is described, achieved through non-SPF housing of the animals and administration of a specific high-fat diet. We describe identification of hepatic and adipose immune cell subsets to recapitulate human immunological conditions by exposing mice to environmental germs.

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