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Capitoli
- 00:04Titolo
- 01:02Vibratome Preparation
- 01:50Tissue Sample Preparation and Sectioning
- 03:19Salivary Gland Section Culture
- 04:01Salivary Gland Section Irradiation and Antibody Staining
- 05:17Results: Representative Characteristics of In Vivo Salivary Gland Section 2D Cultures
- 06:53Conclusion
Using three-dimensional organotypic cultures to visualize morphology and functional markers of salivary glands may provide novel insights into the mechanisms of tissue damage following radiation. Described here is a protocol to section, culture, irradiate, stain, and image 50–90 μm thick salivary gland sections prior to and following exposure to ionizing radiation.
