È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Oxford Centre for Diabetes, Endocrinology and Metabolism,University of Oxford, 2Lund University Diabetes Centre, Unit of Molecular Metabolism, Clinical Research Centre,Malmö University HospitalMalmö, 3Institute of Neuroscience of Physiology, Department of Physiology, Metabolic Research Unit,University of Göteborg, 4Oxford National Institute for Health Research,Biomedical Research Centre, 5Life and Medical Sciences,University of Hertfordshire
Capitoli
- 00:04Titolo
- 00:30Calcium (Ca2+) Imaging Dynamics
- 02:21Microscope Setup
- 03:48Time-Lapse Imaging
- 04:45Numerical Data Analysis
- 06:16Results: Representative Islet Cell Responses to Various Stimuli
- 07:26Conclusion
Här presenterar vi ett protokoll för avbildning och kvantifiering av kalciumdynamik i heterogena cellpopulationer, till exempel pankreasceller. Fluorescerande reportrar levereras i det perifera lagret av celler inom Holmen, som sedan immobiliseras och avbildas, och per cell analys av dynamiken i fluorescensintensiteten utförs.
