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通过将表达的腺病毒注射到小鼠乳腺腺中,模拟乳腺癌

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Modeling Breast Cancer via an Intraductal Injection of Cre-expressing Adenovirus into the Mouse Mammary Gland

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通过将表达的腺病毒注射到小鼠乳腺腺中,模拟乳腺癌

DOI:

10.3791/59502-v

•

06:29 min

•

June 07, 2019

•

Dongxi Xiang², Luwei Tao², Zhe Li²

¹Division of Genetics, Department of Medicine,Brigham and Women’s Hospital, ²Department of Medicine,Harvard Medical School

Capitoli

00:04Titolo
00:34Preoperative Preparation
01:48Intraductal Injection
03:53Results: Key Features of Genetic Manipulations of Mammary Epithelial Cells Based on Intraductal Injection of Ad-Cre
05:25Conclusion

Summary

Traduzione automatica

该协议的目的是描述一种新的乳腺癌建模方法,该方法基于将Cre-表达腺病毒注射到小鼠乳腺的电位内。这种方法允许以时间控制的方式对致癌事件进行细胞型和器官特异性操作。

Tags

Keywords: Breast Cancer Mouse Model Intraductal Injection Cre-expressing Adenovirus Mammary Gland Mammary Epithelial Cells Surgical Procedure Anesthesia Analgesia Hair Removal Disinfection Incision Nipple Removal Hamilton Syringe Dye Ductal Tree

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