/
/
Pasient-avledet heterogen Xenograft modell av bukspyttkjertelen Cancer bruke sebrafisk larver som verter for sammenlignende Drug Assessment
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
1Department of Biochemistry and Molecular Biology, Key Laboratory of Metabolism and Molecular Medicine, Ministry of Education, School of Basic Medical Sciences,Fudan University, 2National Human Genetic Resources Sharing Service Platform (2005DKA21300), 3Fudan University Shanghai Cancer Center, 4Department of Pancreatic Surgery,Fudan University Shanghai Cancer Center, 5Department of Oncology, Shanghai Medical College,Fudan University, 6Pancreatic Cancer Institute,Fudan University
Capitoli
- 00:04Titolo
- 01:28Preparing the Equipment for the Microinjection
- 02:21Preparing Embryos for Transplantation
- 02:56Isolation and Culture of Primary Human Cells from Fresh Surgical Pancreatic Cancer Specimen or Frozen Tissue
- 04:56Labeling the Cells with Lentivirus Expressing Anti-apoptosis Gene BCL2L1 and Different Fluorescent Proteins Separately
- 06:29Injecting Mixed Cell Suspension into the Zebrafish
- 07:10Culture of the Xenografted Zebrafish (zPDX Model), Drug Administrationon the Xenografted Zebrafish, and the Assessment of Tumor Cells/Fibroblasts Viabilities
- 08:11Results: Analysis of the Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae
- 09:12Conclusion
Denne protokollen beskriver optimaliserings prosedyrer i en virus BAS ert dual fluorescens-merket tumor xenograft modell ved hjelp av larvestadiet sebrafisk som verter. Denne heterogene xenograft modellen etterligner vev sammensetningen av bukspyttkjertelkreft mikromiljøet in vivo og fungerer som et mer presist verktøy for å vurdere narkotika responser i personlig zPDX (sebrafisk pasient-avledet xenograft) modeller.
