Rivista
/
Immunologia e infezioni
/
Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

Monitoring Bacterial Colonization and Maintenance on Arabidopsis thaliana Roots in a Floating Hydroponic System

DOI:
10.3791/59517-v

•

09:04 min

•

May 28, 2019

•

, ,
1Department of Microbiology and Immunology,University of North Carolina at Chapel Hill, 2Department of Biology,University of North Carolina at Chapel Hill

Capitoli

  • 00:04Titolo
  • 00:24Preparation of Arabidopsis thaliana Seedlings on a Plastic Mesh
  • 01:36Colonization of Plants in Liquid Bacterial Growth Medium
  • 03:16Bacterial Colonization Maintenance
  • 04:14Collection of Bacteria for Viable Cell Counts
  • 05:19Collection of Intact Plant Roots for Microscopy
  • 06:13Results: Assessment of Bacterial Colonization on Arabidopsis thaliana Roots
  • 08:39Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here we describe a hydroponic plant growth assay to quantify species presence and visualize the spatial distribution of bacteria during initial colonization of plant roots and after their transfer into different growth environments.

Tags

Bacterial ColonizationArabidopsis ThalianaRootsFloating Hydroponic SystemPlant-microbe InteractionsMesh DisksSeed GerminationBacterial Growth MediumColony-forming UnitsGas-permeable FilmPlant Growth Chamber

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

An Orthotopic Model of Serous Ovarian Cancer in Immunocompetent Mice for in vivo Tumor Imaging and Monitoring of Tumor Immune Responses
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Investigating the Effects of Probiotics on Pneumococcal Colonization Using an In Vitro Adherence Assay
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Monitoring the Assembly of a Secreted Bacterial Virulence Factor Using Site-specific Crosslinking
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Assessing Bacterial Invasion of Cardiac Cells in Culture and Heart Colonization in Infected Mice Using Listeria monocytogenes
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Bacterial Leaf Infiltration Assay for Fine Characterization of Plant Defense Responses using the Arabidopsis thaliana-Pseudomonas syringae Pathosystem
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Implementation of a Permeable Membrane Insert-based Infection System to Study the Effects of Secreted Bacterial Toxins on Mammalian Host Cells
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Detection of Enterohemorrhagic Escherichia Coli Colonization in Murine Host by Non-invasive In Vivo Bioluminescence System
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

A High-throughput Shigella-specific Bactericidal Assay
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

Pattern-Triggered Oxidative Burst and Seedling Growth Inhibition Assays in Arabidopsis thaliana
check_url/it/59517?article_type=v&slug=monitoring-bacterial-colonization-maintenance-on-arabidopsis-thaliana

High-throughput Screening of Chemical Compounds to Elucidate Their Effects on Bacterial Persistence

Read Article