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实现干扰反射显微镜,实现无标签、高速微管成像

JoVE Journal
Bioingegneria

This content is Free Access.

JoVE Journal Bioingegneria

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

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实现干扰反射显微镜,实现无标签、高速微管成像

DOI:

10.3791/59520-v

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09:45 min

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August 08, 2019

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Mohammed Mahamdeh², Jonathon Howard

¹Department of Molecular Biophysics and Biochemistry,Yale University, ²Harvard Medical School,Harvard University

Capitoli

00:04Titolo
00:46Microscope Modification and Chamber Preparation
02:32Microscope Alignment
03:43Control: Imaging Stabilized Microtubules
04:43Imaging Microtubule Dynamics
06:28Properly Setting the Aperture Diaphragm Size
08:11Results: IRM Imaging of Microtubules Dynamics and Contrast Enhancement using Image Processing
09:02Conclusion

Summary

Traduzione automatica

本协议是使用体外表面测定在标准荧光显微镜上实施干扰反射显微镜的指南,用于使用体外表面测定对微管进行无标签、高对比度、高速成像。

Tags

Keywords: Interference Reflection Microscopy Label-free High-speed Imaging Microtubules High-contrast DIC Dark Field Fluorescence Microscopy Protein Complexes Nanoparticles 50/50 Mirror Filter Cube High Numerical Aperture Paraffin Film Anti-rhodamine Antibody Poloxamer 407 BRB80

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