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Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis
 

Murine Model of Central Venous Stenosis using Aortocaval Fistula with an Outflow Stenosis

Article DOI: 10.3791/59540-v • 06:17 min • July 11th, 2019
July 11th, 2019 •
1,2,3, 1,2,4,5, 1,2,3, 1,2,3, 1,2,3, 1,2, 1,2, 4, 5, 3, 1,2,6
1Department of Surgery, Yale University, 2Vascular Biology and Therapeutics Program, Yale University, 3Department of Vascular Surgery, University of Tokyo, 4Department of Diagnostic Radiology, Tokai University School of Medicine, 5Department of Cardiovascular Surgery, Tokyo Medical University, 6Department of Vascular Surgery, VA Connecticut Healthcare Systems

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An aortocaval fistula was created by puncturing the murine infra-renal aorta through both walls into the inferior vena cava and was followed by creation of a stenosis in its outflow via partial ligation of the inferior vena cava. This reproducible model can be used to study central venous stenosis.

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Keywords: Central Venous Stenosis Aortocaval Fistula AVF Murine Model C57-Black/6 Mouse Surgical Procedure Aorta Inferior Vena Cava IVC Microneedle Holder Cross-clamp Aortic Puncture Infrarenal IVC 8-0 Polyamide Monofilament Suture 25-gauge Needle
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