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Generation of Organoids from Mouse Extrahepatic Bile Ducts
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Generation of Organoids from Mouse Extrahepatic Bile Ducts

Generation of Organoids from Mouse Extrahepatic Bile Ducts

DOI:
10.3791/59544-v

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09:13 min

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April 23, 2019

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, , , ,
1Departments of Internal Medicine,The University of Michigan, 2Molecular & Integrative Physiology,The University of Michigan

Capitoli

  • 00:04Titolo
  • 00:58Extrahepatic Bile Duct (EHBD) Isolation
  • 02:36Biliary Organoid Culture
  • 03:48EHBD Organoid Passage and Storage
  • 05:01EHBD Organoid Processing for Paraffin Embedding
  • 07:07Results: Representative EHBD Characterization and Analysis
  • 08:24Conclusion

Summary

Traduzione automatica

Traduzione automatica

This protocol describes the production of a mouse extrahepatic bile duct 3-dimensional organoid system. These biliary organoids can be maintained in culture to study cholangiocyte biology. Biliary organoids express markers of both progenitor and biliary cells and are composed of polarized epithelial cells.

Tags

Keywords: OrganoidsExtrahepatic Bile DuctsMurineCholangiopathiesPreclinical ModelsCholangiocyteCell-to-cell InteractionsBile Duct IsolationDissociationBasement MatrixCell Culture

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