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增殖人类抗原特异性CD4+ T细胞使用卡博曲莱辛苏奇尼米酯的定量
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Quantification of Proliferating Human Antigen-specific CD4+ T Cells using Carboxyfluorescein Succinimidyl Ester
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增殖人类抗原特异性CD4+ T细胞使用卡博曲莱辛苏奇尼米酯的定量

DOI:
10.3791/59545-v

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07:00 min

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June 04, 2019

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, , ,
1Immunology and Diabetes Unit,St. Vincent’s Institute of Medical Research, 2Department of Medicine,University of Melbourne, St. Vincent’s Hospital

Capitoli

  • 00:04Titolo
  • 00:41Whole Blood Peripheral Blood Mononuclear Cell (PBMC) Preparation
  • 01:21CD4+ T Cell Preparation
  • 03:16CD4+ T Cell Labeling
  • 04:11Flow Cytometric Analysis
  • 05:31Results: Representative CFSE-labeled, Activated CD4+ T Cell Proliferation
  • 06:21Conclusion

Summary

Traduzione automatica

Traduzione automatica

这里介绍的是一个协议,用于测量增殖CD4+ T细胞,以响应抗原蛋白或肽使用染料稀释。这种测定对稀有抗原特异性T细胞特别敏感,可以进行改性,以利于抗原特异性细胞的克隆。

Tags

CD4+ T CellsProliferationCFSEAntigen-specificQuantificationAutoimmune ConditionsType 1 DiabetesPBMCDensity Gradient CentrifugationCell CultureFlow Cytometry

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