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Preparation of Neutrally-charged, pH-responsive Polymeric Nanoparticles for Cytosolic siRNA Delivery
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Capitoli
- 00:04Titolo
- 00:49Preparation and Characterization of si-NPs (siRNA Nanoparticles)
- 02:00Physicochemical Characterization of si-NPs
- 05:04Determining in vitro Bioactivity of si-NPs
- 06:35Results: Comparison between si-NP 1 and si-NP 2 and siRNA Complexation
- 08:29Conclusion
Methods to prepare and characterize the physicochemical properties and bioactivity of neutrally-charged, pH-responsive siRNA nanoparticles are presented. Criteria for successful siRNA nanomedicines such as size, morphology, surface charge, siRNA loading, and gene silencing are discussed.
