/
/
Droplet Digital TRAP (ddTRAP): Adaptation of the Telomere Repeat Amplification Protocol to Droplet Digital Polymerase Chain Reaction
È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.
Capitoli
- 00:04Titolo
- 00:27Cell Lysis and Telomerase Extension Reaction
- 01:43Droplet Digital PCR Setup
- 03:21Detection of Telomerase Extension Products and Data Analysis
- 05:03Results: Telomerase Activity
- 05:51Conclusion
We successfully converted the standard telomere repeat amplification protocol (TRAP) assay to be employed in droplet digital polymerase chain reactions. This new assay, called ddTRAP, is more sensitive and quantitative, allowing for better detection and statistical analysis of telomerase activity within various human cells.
