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Journal / Biochemistry / Enrichment of Native Lipoprotein Particles with…
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Biochemistry

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JoVE Journal Biochemistry
Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate
 

Enrichment of Native Lipoprotein Particles with microRNA and Subsequent Determination of Their Absolute/Relative microRNA Content and Their Cellular Transfer Rate

Article DOI: 10.3791/59573-v • 11:13 min • May 9th, 2019
May 9th, 2019 •
1, 2, 1, 1, 2
1Center for Pathobiochemistry and Genetics, Institute of Medical Chemistry and Pathobiochemistry, Medical University Vienna, 2School of Medical Engineering and Applied Social Sciences, University of Applied Sciences Upper Austria

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Here, a quantitative real-time polymerase chain reaction-based protocol is presented for the determination of the native micro-RNA content (absolute/relative) of lipoprotein particles. In addition, a method for increasing the micro-RNA level, as well as a method for determining the cellular uptake rate of lipoprotein particles, is demonstrated.

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Keywords: Lipoprotein Particles MicroRNA Cellular Uptake Delipidation Enrichment Relipidation Bradford Protein Assay Reconstitution Spermine Synthetic MicroRNA
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