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Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease
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Biologia
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JoVE Journal Biologia
Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease

Fabrication of Amyloid-β-Secreting Alginate Microbeads for Use in Modelling Alzheimer’s Disease

DOI:
10.3791/59597-v

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06:52 min

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July 06, 2019

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, , ,
1Division of Pharmacy and Optometry, School of Health Studies, Faculty of Biology, Medicine and Health,University of Manchester, 2Division of Neuroscience and Experimental Psychology, School of Biological Sciences, Faculty of Biology, Medicine and Health,University of Manchester

Capitoli

  • 00:04Titolo
  • 01:02Setting Up the Encapsulation System
  • 02:16Microbeads Fabrication
  • 04:10Testing and Use of Microbeads
  • 04:57Results: Testing of Amyloid–Secreting
  • 06:31Conclusion

Summary

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This protocol illustrates a cell encapsulation method by rapid physical gelation of alginate to immobilize cells. Obtained microbeads allow controlled and sustained secretion of amyloid-β over time and can be used to study the effects of secreted amyloid-β in in vitro and in vivo models.

Tags

AmyloidAlginate MicrobeadsAlzheimer’s DiseaseCell EncapsulationControlled ReleaseBiomolecule ReleaseCell SuspensionAlginate SolutionEncapsulatorGelation Bath

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