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活鼠中多萨尔希波坎卡尔CA1的纵向双光成像
JoVE Journal
Comportamento
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JoVE Journal Comportamento
Longitudinal Two-Photon Imaging of Dorsal Hippocampal CA1 in Live Mice
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活鼠中多萨尔希波坎卡尔CA1的纵向双光成像

DOI:
10.3791/59598-v

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09:34 min

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June 19, 2019

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, , , , , ,
1Dept. of Stress Neurobiology and Neurogenetics,Max Planck Institute of Psychiatry, 2Graduate School of Systemic Neurosciences,Ludwig Maximilians University, 3Max Planck Florida Institute for Neuroscience, 4Dept. of Neurobiology,Weizmann Institute of Science

Capitoli

  • 00:04Titolo
  • 01:04Preparation of the Imaging Cannula
  • 02:11Implantation of the Imaging Cannula Over the Dorsal Hippocampus
  • 05:32Imaging Preparation
  • 06:16Cannula Imaging
  • 07:10Results: Longitudinal Imaging of Dendritic Structure and Dendritic Spines Dynamics
  • 08:50Conclusion

Summary

Traduzione automatica

Traduzione automatica

这种方法描述了一种慢性制剂,允许光学访问活小鼠的海马区。这种制剂可用于在几周内对神经元结构可塑性和活动诱发细胞可塑性进行纵向光学成像。

Tags

Two-photon ImagingDorsal HippocampusCA1Live MiceLongitudinalMemory FormationMemory RecallAlzheimer’s DiseaseSurvival SurgeryImaging CannulaUV CuringDental DrillLambdaBregmaLidocainePeriosteum

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