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原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Functionalization of Atomic Force Microscope Cantilevers with Single-T Cells or Single-Particle for Immunological Single-Cell Force Spectroscopy
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原子力显微镜罐体功能化,具有单T细胞或单粒子免疫单细胞力光谱

DOI:
10.3791/59609-v

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10:06 min

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July 10, 2019

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, , ,
1State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine,Peking Union Medical College, 2Institute for Immunology, School of Medicine,Tsinghua University

Capitoli

  • 00:04Titolo
  • 01:17AFM Cantilever Preparation
  • 02:36Attaching Single T-cells to the Cantilever
  • 04:58Force Spectroscopy of Single-pair T-cell/Dendritic Cell Interaction
  • 05:34Cantilever Functionalization with Single Polystyrene Beads
  • 07:44Results: Measuring Cell-Cell Adhesions
  • 09:30Conclusion

Summary

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我们提出了一种协议,用单个T细胞和珠子粒子使原子力显微镜(AFM)的吊带功能化,用于免疫学研究。显示了通过AFM探测单对T细胞树突状细胞结合的过程,并监测宏噬细胞对单个固体颗粒的实时细胞响应,并采用荧光成像。

Tags

Atomic Force MicroscopeCantilever FunctionalizationSingle T CellSingle ParticleImmunological Force SpectroscopyDendritic Cell-T Cell InteractionCell-surface InteractionBiocompatible GlueIL-2Sample PreparationCalibrationLiving Cell Environment

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