Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

George  S. Karagiannis; Jessica  M. Pastoriza; Lucia Borriello; Rojin Jafari; Anouchka Coste; John  S. Condeelis; Maja H. Oktay; David Entenberg

doi:10.3791/59633

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利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境

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Assessing Tumor Microenvironment of Metastasis Doorway-Mediated Vascular Permeability Associated with Cancer Cell Dissemination using Intravital Imaging and Fixed Tissue Analysis

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利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境

DOI:

10.3791/59633-v

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09:42 min

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June 26, 2019

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George S. Karagiannis^2,3, Jessica M. Pastoriza^2,4, Lucia Borriello², Rojin Jafari², Anouchka Coste^2,4, John S. Condeelis^2,3,4, Maja H. Oktay^2,3,5, David Entenberg^2,3

¹Department of Anatomy and Structural Biology,Albert Einstein College of Medicine, ²Gruss-Lipper Biophotonics Center,Albert Einstein College of Medicine, ³Integrated Imaging Program,Albert Einstein College of Medicine, ⁴Department of Surgery,Montefiore Medical Center, ⁵Department of Pathology,Montefiore Medical Center

Capitoli

00:04Titolo
00:55Transplantation of Syngeneic Breast Tumors into Mouse Hosts with Fluorescent Macrophages
02:38Preparation of Mouse for Imaging
04:25Evaluation of Extravascular Dextran Using Fixed Tissue Analysis
07:10Results: Visualization of Vasculature in Healthy and Diseased Tissues and TMEM-Mediated Vascular Permeability
08:56Conclusion

Summary

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我们描述了两种方法,用于评估与转移(TMEM)的肿瘤微环境(TMEM)和癌细胞内溢相关的瞬态血管渗透性,使用静脉注射高分子量(155 kDa)dextran小鼠。这些方法包括活体动物的活体成像和使用免疫荧光的固定组织分析。

利用生命内成像和固定组织分析评估与癌细胞传播相关的转移门道介导血管渗透性的肿瘤微环境

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