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In Vitro ELISA Test to Evaluate Rabies Vaccine Potency
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JoVE Journal Immunologia e infezioni
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

DOI:
10.3791/59641-v

•

09:04 min

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May 11, 2020

•

,
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus,Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

Capitoli

  • 00:04Introduction
  • 01:08Microplate Sensitization
  • 02:07Microplate Passivation
  • 02:33ELISA Assay
  • 06:44Results: Analysis of the ELISA Test
  • 08:00Conclusion

Summary

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Here we describe an indirect ELISA sandwich immunocapture to determine the immunogenic glycoprotein contents in rabies vaccines. This test uses a neutralizing Monoclonal Antibody (mAb-D1) recognizing glycoprotein trimers. It is an alternative to the in vivo NIH test to follow the consistency of vaccine potency during production.

Tags

ELISARabies VaccinePotencyIn VitroAnimal TestingNH TestImmunogenic GlycoproteinSensitivityIn VivoManufacturersNational Control LaboratoriesPersonal Protective EquipmentDecontaminationMonoclonal AntibodyPassivation BufferWashing BufferReference VaccineDilution

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