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In Vitro ELISA test til vurdering af rabiesvaccine potens
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JoVE Journal Immunologia e infezioni
In Vitro ELISA Test to Evaluate Rabies Vaccine Potency

In Vitro ELISA test til vurdering af rabiesvaccine potens

DOI:
10.3791/59641-v

•

09:04 min

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May 11, 2020

•

,
1Unit Antiviral Strategies, OIE Reference Laboratories for Rift Valley Fever Virus & Crimean Congo Hemorrhagic Fever Virus,Institut Pasteur, 2Institut Pasteur de Guinée, Conakry

Capitoli

  • 00:04Introduction
  • 01:08Microplate Sensitization
  • 02:07Microplate Passivation
  • 02:33ELISA Assay
  • 06:44Results: Analysis of the ELISA Test
  • 08:00Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her beskriver vi en indirekte ELISA sandwich immunfangst til bestemmelse af det immunogene glycoprotein indhold i rabiesvacciner. Denne test bruger en neutraliserende Monoklonal Antistof (mAb-D1) genkende glycoprotein trimers. Det er et alternativ til in vivo NIH-testen for at følge konsistensen af vaccinestyrken under produktionen.

Tags

ELISARabies VaccinePotencyIn VitroAnimal TestingNH TestImmunogenic GlycoproteinSensitivityIn VivoManufacturersNational Control LaboratoriesPersonal Protective EquipmentDecontaminationMonoclonal AntibodyPassivation BufferWashing BufferReference VaccineDilution

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