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Journal / Genetics / Designing Automated, High-throughput, Continuous…
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Genetics

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JoVE Journal Genetics
Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER
 

Designing Automated, High-throughput, Continuous Cell Growth Experiments Using eVOLVER

Article DOI: 10.3791/59652-v • 07:26 min • May 19th, 2019
May 19th, 2019 •
1,2, 1,2, 1,3, 1,2, 4, 1,2,5
1Biological Design Center, Boston University, 2Department of Biomedical Engineering, Boston University, 3Program in Molecular Biology, Cell Biology and Biochemistry, Boston University, 4Department of Bioengineering, Rice University, 5Wyss Institute for Biologically Inspired Engineering, Harvard University

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The eVOLVER framework enables high-throughput continuous microbial culture with high resolution and dynamic control over experimental parameters. This protocol demonstrates how to apply the system to conduct a complex fitness experiment, guiding users on programming automated control over many individual cultures, measuring, collecting, and interacting with experimental data in real-time.

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Keywords: EVOLVER Automated Cell Growth Experiments High-throughput Continuous Culture Aerobic Culturing Bacteria Yeast Anaerobic Fermentation Mammalian Cells Media Preparation Sterilization Fluid Lines Pumps Touchscreen Setup Python Customization Sensors Visual Guide
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