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Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils

DOI:
10.3791/59667-v

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07:15 min

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January 21, 2020

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,
1Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre,University Health Network, 2Faculty of Dentistry,University of Toronto

Capitoli

  • 00:04Titolo
  • 01:15Ligature Installation
  • 03:29Sample Collection
  • 05:05Antibody Staining for Flow Cytometric Analysis
  • 06:03Results: FACS and Alveolar Bone Loss Analyses
  • 06:37Conclusion

Summary

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This article presents a protocol for establishing a ligature-induced model of murine periodontitis involving multiple maxillary molars, resulting in larger areas of the involved gingival tissue and bone for subsequent analysis as well as reduced animal usage. A technique to assess oral neutrophils in a manner analogous to human subjects is also described.

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Keywords: Ligature-inducedMurine PeriodontitisOral NeutrophilsMicrosurgical InstrumentsSurgical MicroscopeBraided Silk SutureMaxillary MolarsGingival SulcusSurgeon’s Knot

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