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用于口腔中性粒细胞病评价的鼠期牙周炎的强结合诱导模型
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Robust Ligature-Induced Model of Murine Periodontitis for the Evaluation of Oral Neutrophils
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用于口腔中性粒细胞病评价的鼠期牙周炎的强结合诱导模型

DOI:
10.3791/59667-v

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07:15 min

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January 21, 2020

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,
1Department of Dental Oncology and Maxillofacial Prosthetics, Princess Margaret Cancer Centre,University Health Network, 2Faculty of Dentistry,University of Toronto

Capitoli

  • 00:04Titolo
  • 01:15Ligature Installation
  • 03:29Sample Collection
  • 05:05Antibody Staining for Flow Cytometric Analysis
  • 06:03Results: FACS and Alveolar Bone Loss Analyses
  • 06:37Conclusion

Summary

Traduzione automatica

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本文提出了建立涉及多个上颌骨的月牙病结扎诱导模型的协议，从而在涉及牙龈组织和骨骼的较大区域进行后续分析，并减少动物使用。还介绍了一种以类似于人类受试者的方式评估口服中性粒细胞的技术。

Tags

Ligature-inducedMurine PeriodontitisOral NeutrophilsMicrosurgical InstrumentsSurgical MicroscopeBraided Silk SutureMaxillary MolarsGingival SulcusSurgeon’s Knot

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