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Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery
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Neuroscienze
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JoVE Journal Neuroscienze
Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery

Delivery of Antibodies into the Murine Brain via Convection-enhanced Delivery

DOI:
10.3791/59675-v

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08:22 min

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July 18, 2019

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, , ,
1Institute of Laboratory Animal Science,University of Zurich

Capitoli

  • 00:04Titolo
  • 00:49Step Catheter Preparation
  • 02:29Step Catheter Testing
  • 04:46Antibody Solution Convection-enhanced Delivery (CED) into the Murine Brain
  • 06:34Results: Representative CED Infusion Analyses
  • 07:40Conclusion

Summary

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Convection-enhanced delivery (CED) is a method enabling effective delivery of therapeutics into the brain by direct perfusion of large tissue volumes. The procedure requires the use of catheters and an optimized injection procedure. This protocol describes a methodology for CED of an antibody into a mouse brain.

Tags

Keywords: Convection-enhanced DeliveryAntibody DeliveryBrainMurineStep CatheterBlood-brain BarrierFused Silica CapillaryCyanoacrylate AdhesiveStereotactic RobotAgarose GelTrypan Blue

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