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循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估
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Micromanipulation of Circulating Tumor Cells for Downstream Molecular Analysis and Metastatic Potential Assessment
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循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

DOI:
10.3791/59677-v

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05:17 min

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May 14, 2019

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, , , ,
1Cancer Metastasis Laboratory, Department of Biomedicine,University of Basel and University Hospital Basel, 2SIB Swiss Institute of Bioinformatics

Capitoli

  • 00:04Titolo
  • 00:28Circulating Tumor Cell (CTC) Micromanipulation and Single-Cell Picking
  • 03:31Results: Representative Downstream Molecular Analysis and Metastatic Potential Assessment of Single-Cell Picked CTC
  • 04:42Conclusion

Summary

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在这里, 我们提出了一个综合的工作流程, 以确定表型和分子特征的循环肿瘤细胞 (Ctc)。我们将单个和聚集 Ctc 的活体免疫染色和机器人微操作与单细胞技术结合起来, 对转移种子能力进行下游分析和评估。

Tags

MicromanipulationCirculating Tumor CellsCTCSingle Cell AnalysisMolecular AnalysisMetastatic PotentialMicromanipulatorCell SeparationCell PickingCell DepositionDownstream AnalysisUltra-low Attachment Plate384-well PlateCell Culture Medium

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