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Klargøring af muse retinale Cryo-sektioner til immun histokemi
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JoVE Journal Neuroscienze
Preparation of Mouse Retinal Cryo-sections for Immunohistochemistry

Klargøring af muse retinale Cryo-sektioner til immun histokemi

DOI:
10.3791/59683-v

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05:25 min

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July 01, 2019

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, , ,
1Department of Biomedical Sciences,University of Pennsylvania School of Veterinary Medicine, 2Division Experimental Retinal Therapies, Department of Clinical Sciences and Advanced Medicine,University of Pennsylvania School of Veterinary Medicine

Capitoli

  • 00:04Titolo
  • 00:45Mouse Eye Enucleation, Eye Cup Dissection, and Embedding
  • 03:08Sectioning Using a Cryostat
  • 03:51Results: Immunohistochemistry of Mouse Retinal Cryo-sections
  • 05:01Conclusion

Summary

Traduzione automatica

Traduzione automatica

Denne rapport beskriver omfattende metoder til at forberede frosne mus nethinden sektioner for Immunhistokemi (IHC). Beskrevne metoder omfatter dissektion af okulær posterior kop, PARAFORMALDEHYD fiksering, indlejring i optimal skæring temperatur (OCT) medier og vævs orientering, skæring og immun farvning.

Tags

Keywords: Mouse RetinaRetinal DissectionImmunohistochemistryCryo-sectionEye EnucleationEye FixationCornea RemovalLens ExtractionRetina IsolationCryo-protectionSnap Freezing

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