/

/

近红外荧光和高分辨率扫描测量啮齿类动物大脑中蛋白质的表达

JoVE Journal
Neuroscienze

È necessario avere un abbonamento a JoVE per visualizzare questo. Accedi o inizia la tua prova gratuita.

JoVE Journal Neuroscienze

Using Near-infrared Fluorescence and High-resolution Scanning to Measure Protein Expression in the Rodent Brain

Please note that all translations are automatically generated. Click here for the English version.

近红外荧光和高分辨率扫描测量啮齿类动物大脑中蛋白质的表达

DOI:

10.3791/59685-v

•

06:04 min

•

May 23, 2019

•

Brianna Kimmelmann-Shultz, Negin Mohmammadmirzaei, Jeffrey Caplan, Dayan Knox

¹Department of Psychological and Brain Sciences,University of Delaware, ²Plant and Soil Sciences, Delaware Biotechnology Institute,University of Delaware

Capitoli

00:04Titolo
00:45Single Immunohistochemical Reaction
02:45Imaging and Protein Expression Analysis
04:14Results: Detection and Measurement of Protein Expression in the Rodent Brain
05:30Conclusion

Summary

Traduzione automatica

在这里, 我们提出了一个协议, 使用近红外染料结合免疫组织化学和高分辨率扫描来检测大脑区域的蛋白质。

Tags

Keywords: Near-infrared Fluorescence High-resolution Scanning Protein Expression Rodent Brain Immunohistochemistry Molecular Signaling Pathways AMPA/NMDA Ratio Cryostat Paraformaldehyde TBS Primary Antibody Secondary Antibody Near-infrared Scanning

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

同时在啮齿动物脑功能磁共振成像和电

一个快速高分辨率的荧光成像的方法在半厚的脑片

使用近红外光谱MazeSuite和功能研究学习空间导航

高分辨率实时成像发展中的神经上皮细胞的行为

大脑切片生物素化：一个<em>前体内</em>方法来衡量地区特有的质膜蛋白贩运成年神经元

在大鼠脑组织用荧光活化细胞分选，以检查细胞类型特异性基因表达

在脑切片检测轴突本地化mRNAs的高空间分辨率<em>原位</em>杂交

一个简单的方法来衡量在改建悬赏寻求行为使用<em>果蝇</em

啮齿动物脑 Microinjections 的个性化针

利用功能近红外光谱进行超扫描实验

Read Article