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A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry
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Biologia
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JoVE Journal Biologia
A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

A Uniform Shear Assay for Human Platelet and Cell Surface Receptors via Cone-plate Viscometry

DOI:
10.3791/59704-v

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04:32 min

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June 05, 2019

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, ,
1Aflac Cancer and Blood Disorders Center,Children’s Healthcare of Atlanta, 2Department of Pediatrics,Emory University School of Medicine

Capitoli

  • 00:04Titolo
  • 00:34Platelet Isolation
  • 01:15Ligand and Uniform Shear Treatment and Surface Marker Detection
  • 02:49Results: Representative Platelet Activation Marker Expression
  • 04:01Conclusion

Summary

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We describe an in-solution method to apply uniform shear to platelet surface receptors using cone-plate viscometry. This method may also be used more broadly to apply shear to other cell types and cell-fragments and need not target a specific ligand-receptor pair.

Tags

Keywords: Uniform Shear AssayCone-plate ViscometryPlateletCell Surface ReceptorsShearFlow CytometryFluorescent MarkersPeripheral BloodPlatelet-rich PlasmaAntibody TreatmentShear RateShear DurationSurface Markers

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