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Capitoli
- 00:04Titolo
- 00:35Tools and Solutions for Injections
- 01:30Preparation of the Injection Station
- 02:28Preparation of the Fish for Intrathoracic Injection
- 03:04Microinjection into the Pericardium
- 04:07Results: Validation and Analysis of the Intrathoracic Injection Method
- 06:35Conclusion
This method relies on the injection of 0.5−3 μL of solution into the thorax of adult zebrafish. The procedure efficiently delivers proteins and chemical compounds into the proximity of the zebrafish heart without damaging the organ. The approach is suitable for testing effects of exogenous factors on various tissues of the heart.
