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Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages

DOI:
10.3791/59731-v

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06:47 min

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May 24, 2019

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1Division of Immunology, Allergy, and Rheumatology, Department of Internal Medicine, College of Medicine,University of Cincinnati

Capitoli

  • 00:04Titolo
  • 00:36Apoptotic Thymocyte Carboxyfluorescein Succinimidyl Ester (CFSE)-labeling
  • 02:39Peritoneal Macrophage Preparation
  • 03:53Peritoneal Macrophage: Apoptotic Thymocyte Co-culture
  • 04:36Results: Representative Macrophage Phagocytosis and Efferocytosis Analyses
  • 06:12Conclusion

Summary

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Here, we present a protocol to prepare apoptotic thymocytes and peritoneal macrophages and analyze the efficiency of efferocytosis and the specific inhibitor-mediated blocking of apoptotic thymocytes engulfment. This protocol has a broad application in cell-mediated clearance of other particles including artificial beads and bacteria.

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Keywords: Apoptotic CellsThymocyte EngulfmentMacrophagesPhagocytic Cell TypesApoptotic Cell BindingCell DebrisCell ClearanceCFSE LabelingStaurosporinePeritoneal MacrophagesThioglycollateCell Isolation

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