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巨噬细胞吞噬胸腺细胞吞噬的实验分析
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Experimental Analysis of Apoptotic Thymocyte Engulfment by Macrophages
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巨噬细胞吞噬胸腺细胞吞噬的实验分析

DOI:
10.3791/59731-v

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06:47 min

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May 24, 2019

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1Division of Immunology, Allergy, and Rheumatology, Department of Internal Medicine, College of Medicine,University of Cincinnati

Capitoli

  • 00:04Titolo
  • 00:36Apoptotic Thymocyte Carboxyfluorescein Succinimidyl Ester (CFSE)-labeling
  • 02:39Peritoneal Macrophage Preparation
  • 03:53Peritoneal Macrophage: Apoptotic Thymocyte Co-culture
  • 04:36Results: Representative Macrophage Phagocytosis and Efferocytosis Analyses
  • 06:12Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里, 我们提出了一个方案, 以编制凋亡胸腺细胞和腹膜巨噬细胞, 并分析了细胞内吞的效率和特定的抑制介导的抑制凋亡胸腺细胞吞没。该协议在包括人工珠子和细菌在内的其他颗粒的细胞介导清除中有着广泛的应用。

Tags

Apoptotic CellsThymocyte EngulfmentMacrophagesPhagocytic Cell TypesApoptotic Cell BindingCell DebrisCell ClearanceCFSE LabelingStaurosporinePeritoneal MacrophagesThioglycollateCell Isolation

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