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The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
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Genetica
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JoVE Journal Genetica
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior

DOI:
10.3791/59732-v

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08:12 min

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June 12, 2019

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1Department of Biology,Villanova University

Capitoli

  • 00:04Titolo
  • 00:56In Vitro Invasion Assay
  • 05:55Imaging and Analysis
  • 06:27Results: Analysis of the In Vitro Invasion Assay
  • 07:22Conclusion

Summary

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The in vitro cell invasion assay is used to measure the potential of cancer metastasis by quantifying the cellular potential for invasion and migration using cell culture inserts containing protein matrix. Cells are challenged to migrate through the protein matrix and a porous membrane, towards a chemoattractant, and then quantified by light microscopy.

Tags

Keywords: Mouse Mammary Tumor CellsIn Vitro Invasion AssayCell MigrationCell InvasionOncogenic Cell BehaviorBoyden ChamberPorous MembraneCell CultureCell SuspensionCell ConcentrationSerum-free DMEM

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