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小鼠乳腺肿瘤细胞在体外入侵测定中作为致癌细胞行为的测量
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Genetica
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JoVE Journal Genetica
The Use of Mouse Mammary Tumor Cells in an In Vitro Invasion Assay as a Measure of Oncogenic Cell Behavior
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小鼠乳腺肿瘤细胞在体外入侵测定中作为致癌细胞行为的测量

DOI:
10.3791/59732-v

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08:12 min

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June 12, 2019

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,
1Department of Biology,Villanova University

Capitoli

  • 00:04Titolo
  • 00:56In Vitro Invasion Assay
  • 05:55Imaging and Analysis
  • 06:27Results: Analysis of the In Vitro Invasion Assay
  • 07:22Conclusion

Summary

Traduzione automatica

Traduzione automatica

体外细胞入侵测定使用含有蛋白质基质的细胞培养插入物,通过量化细胞入侵和迁移的可能性来测量癌症转移的潜能。细胞面临的挑战是通过蛋白质基质和多孔膜迁移,向化学吸引剂迁移,然后通过光显微镜进行量化。

Tags

Keywords: Mouse Mammary Tumor CellsIn Vitro Invasion AssayCell MigrationCell InvasionOncogenic Cell BehaviorBoyden ChamberPorous MembraneCell CultureCell SuspensionCell ConcentrationSerum-free DMEM

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