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Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis
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Biologia dello sviluppo
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JoVE Journal Biologia dello sviluppo
Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

Development of Targeting Induced Local Lesions IN Genomes (TILLING) Populations in Small Grain Crops by Ethyl Methanesulfonate Mutagenesis

DOI:
10.3791/59743-v

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08:36 min

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July 16, 2019

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, , , , , ,
1Department of Plant Science and Landscape Architecture,University of Maryland, College Park

Capitoli

  • 00:04Titolo
  • 00:43Preparation of Dosage Curve for Effective Mutagenesis
  • 03:09Cel-1 Assay for Genetic Characterization of Mutants
  • 06:03Results: EMS Mutation
  • 07:37Conclusion

Summary

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Described is a protocol for developing a Targeting Induced Local Lesions IN Genomes (TILLING) population in small grain crops with use of ethyl methanesulfonate (EMS) as a mutagen. Also provided is a protocol for mutation detection using the Cel-1 assay.

Tags

TILLINGMutagenesisEMSSmall Grain CropsFunctional GenomicsGene DiscoveryCel-1 AssayMutation DetectionDosage OptimizationM2 Plants

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