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多端根结核细胞迁移的实时监测

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Ricerca sul cancro

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JoVE Journal Ricerca sul cancro

Real-Time Monitoring of Human Glioma Cell Migration on Dorsal Root Ganglion Axon-Oligodendrocyte Co-Cultures

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多端根结核细胞迁移的实时监测

DOI:

10.3791/59744-v

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06:51 min

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December 13, 2019

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John P. Zepecki, Kristin M. Snyder, Nikos Tapinos³

¹Molecular Neuro-oncology Laboratory,Brown University, Rhode Island Hospital, ²University of Minnesota, College of Veterinary Medicine, ³Department of Neurosurgery,Brown University

Capitoli

00:04Titolo
00:36Compartmented Culture of Rat Dorsal Root Ganglia (DRGs), Oligodendrocytes (OPCs) and Human Glioma Cells (hGCs)
03:17Culture in the Compartmented Chamber
04:14Results: hGCs in DRG Axons
05:52Conclusion

Summary

Traduzione automatica

在这里，我们提出了一个前活体混合单层培养系统，用于实时研究人类胶质瘤细胞（hGC）迁移。该模型提供了观察hGCs与隔板腔内骨髓和非骨髓化斧子之间的相互作用的能力。

Tags

Keywords: Real-time Monitoring Human Glioma Cell Migration Dorsal Root Ganglion Axon-oligodendrocyte Co-cultures Ex Vivo Co-culture System Cellular And Molecular Mechanisms In Vitro Drug Efficacy Testing Compartmented Culture Dishes Collagen Coating Ammonium Hydroxide Silicone Grease Blunt-tip Needle Supplemented Neuronal Basal Medium

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