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Chapters
- 00:04Title
- 00:52Expression of Wild-type BM3 (Bacterial Cytochrome P450 Enzyme)
- 02:10Purification of BM3
- 05:35Extraction of EDPs (Epoxydocosapentaenoic Acids)
- 07:23Esterification of EDPs, Separation of 16(S), 17(R)- and 19(S),20(R)-EDP, and Saponification of Esters
- 11:17Results: Chromatogram and 1H-NMR Spectra
- 12:13Conclusion
Vi præsenterer en metode, der er nyttig til storstilet enzymatisk syntese og rensning af specifikke enantiomerer og regioisomerer af Epoxider af arachidonsyre (AA), docosahexaensyre (DHA) og eicosapentaensyre Acid (EPA) med brug af en bakteriel cytokrom P450-enzym (BM3).
