Rivista
/
Biochimica
/
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System
JoVE Journal
Biochimica
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biochimica
A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

A Double Humanized BLT-mice Model Featuring a Stable Human-Like Gut Microbiome and Human Immune System

DOI:
10.3791/59773-v

•

00:07 min

•

August 30, 2019

•

, , ,
1Nebraska Center for Virology, 2School of Biological Sciences,University of Nebraska-Lincoln, 3Department of Food Science and Technology,University of Nebraska-Lincoln

Capitoli

  • 00:04Titolo
  • 00:41Bone Marrow, Liver, Thymus-Humanized (hu-BLT) Mouse Generation
  • 03:43Human Immune Cell Level Evaluation and Fresh Fecal Sample Collection
  • 05:20Fecal Transplant
  • 06:05Results: Representative Double Humanized BLT-Mouse Gut Microbiome Analyses
  • 07:02Conclusion

Summary

Traduzione automatica

Traduzione automatica

We describe a novel method for generating double humanized BLT-mice that feature a functional human immune system and a stable engrafted human-like gut microbiome. This protocol can be followed without the need for germ-free mice or gnotobiotic facilities.

Tags

Double Humanized MiceHuman Gut MicrobiomeHuman Immune SystemBiomedical ResearchHematopoietic Stem CellsTransplantationSurgery Procedure

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Thermostabilization, Expression, Purification, and Crystallization of the Human Serotonin Transporter Bound to S-citalopram

A Method for Measuring Metabolism in Sorted Subpopulations of Complex Cell Communities Using Stable Isotope Tracing

A Modified Yeast-one Hybrid System for Heteromeric Protein Complex-DNA Interaction Studies

A Guide to Production, Crystallization, and Structure Determination of Human IKK1/α

Assessing the Viability of a Synthetic Bacterial Consortium on the In Vitro Gut Host-microbe Interface

Manipulating Living Cells to Construct Stable 3D Cellular Assembly Without Artificial Scaffold

Sampling and Pretreatment of Tooth Enamel Carbonate for Stable Carbon and Oxygen Isotope Analysis

Calibration-free In Vitro Quantification of Protein Homo-oligomerization Using Commercial Instrumentation and Free, Open Source Brightness Analysis Software

Untargeted Liquid Chromatography-Mass Spectrometry-Based Metabolomics Analysis of Wheat Grain

Analysis and Specification of Starch Granule Size Distributions

Read Article