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在定义良好的剪切应力下单细胞电电流的电生理记录
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Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Electrophysiological Recordings of Single-cell Ion Currents Under Well-defined Shear Stress
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在定义良好的剪切应力下单细胞电电流的电生理记录

DOI:
10.3791/59776-v

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07:17 min

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August 02, 2019

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1Department of Medicine, Division of Pulmonary, Critical Care, Sleep and Allergy,University of Illinois at Chicago

Capitoli

  • 00:04Titolo
  • 00:29Assembly of the Modified Parallel Plate Flow Chamber
  • 01:43Cell Preparation and Seeding into the MPP Flow Chamber
  • 03:43Controlling Shear Stress to the MPP Flow Chamber for Electrophysiological Recordings
  • 05:46Results: Example of Leak Subtraction for Accurate Analysis of Mechano-activated Kir Current
  • 06:43Conclusion

Summary

Traduzione automatica

Traduzione automatica

该协议的目的是描述一个经过修改的平行板流室,用于研究通过剪切应力实时激活美能敏感子孔通道。

Tags

Keywords: Electrophysiological RecordingsSingle-cell Ion CurrentsShear StressParallel Plate Flow ChamberMechanosensitive Ion ChannelsSilicone ElastomerCover GlassCell CultureElectrophysiological BAF SolutionCell Adhesion

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