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李斯特菌单细胞基因感染后小鼠脾脏原位干扰素伽马生产的成像
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection
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李斯特菌单细胞基因感染后小鼠脾脏原位干扰素伽马生产的成像

DOI:
10.3791/59819-v

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09:11 min

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July 16, 2019

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, , ,
1The Kennedy Institute of Rheumatology,The University of Oxford

Capitoli

  • 00:04Titolo
  • 01:03Listeria monocytogenes (LM) Infection and Brefeldin A (BFA) Treatment
  • 02:02Spleen Harvesting, Fixation with Paraformaldehyde (PFA), Freezing and Sectioning
  • 04:41Immunofluorescent Staining and Imaging
  • 06:44Results: IFNγ Production
  • 08:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

在这里,我们描述了一个简单的共聚焦成像方法,以可视化细胞在原位定位,在鼠次淋巴器官中分泌细胞因子干扰素伽马的细胞。该协议可以扩展用于不同组织中其他细胞因子的可视化。

Tags

Keywords: In SituInterferon GammaListeria MonocytogenesMouse SpleenCytokine VisualizationMicroenvironmentLM OVA CultureIntravenous InjectionBFAEuthanizationSpleen DissectionFixative Solution

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