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Billeddannelse af in situ-interferon gamma produktion i mus milt efter infektion med Listeria monocytogenes
JoVE Journal
Immunologia e infezioni
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JoVE Journal Immunologia e infezioni
Imaging of In Situ Interferon Gamma Production in the Mouse Spleen following Listeria monocytogenes Infection

Billeddannelse af in situ-interferon gamma produktion i mus milt efter infektion med Listeria monocytogenes

DOI:
10.3791/59819-v

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09:11 min

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July 16, 2019

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, , ,
1The Kennedy Institute of Rheumatology,The University of Oxford

Capitoli

  • 00:04Titolo
  • 01:03Listeria monocytogenes (LM) Infection and Brefeldin A (BFA) Treatment
  • 02:02Spleen Harvesting, Fixation with Paraformaldehyde (PFA), Freezing and Sectioning
  • 04:41Immunofluorescent Staining and Imaging
  • 06:44Results: IFNγ Production
  • 08:19Conclusion

Summary

Traduzione automatica

Traduzione automatica

Her beskriver vi en simpel Konfokal billedbehandlings metode for at visualisere in situ-lokalisering af celler, der udskiler cytokininterferon gamma i murine sekundære lymfoide organer. Denne protokol kan udvides til visualisering af andre cytokiner i forskellige væv.

Tags

Keywords: In SituInterferon GammaListeria MonocytogenesMouse SpleenCytokine VisualizationMicroenvironmentLM OVA CultureIntravenous InjectionBFAEuthanizationSpleen DissectionFixative Solution

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