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Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
JoVE Journal
Biochimica
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JoVE Journal Biochimica
Characterization at the Molecular Level using Robust Biochemical Approaches of a New Kinase Protein
DOI:

11:23 min

June 30, 2019

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Capitoli

  • 00:04Titolo
  • 01:24Transient Transfections
  • 03:21Lysis
  • 05:17Immunoprecipitation
  • 06:59Coimmunoprecipitation Analyses
  • 08:33Results: Synthesized LIMK2-1 Protein, Its Interaction with Kinase ROCK, its Kinase Activity
  • 10:10Conclusion

Summary

Traduzione automatica

We characterized a new kinase protein using robust biochemical approaches: Western Blot analysis with a dedicated specific antibody on different cell lines and tissues, interactions by coimmunoprecipitation experiments, kinase activity detected by Western Blot using a phospho-specific antibody and by γ[32P] ATP labeling.

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